Egg freezing has become a practical strategy for women to preserve fertility and collect mature eggs. However, oocytes are vulnerable to freezing damage, and many organelles and structures may be affected, which also affects the survival rate of eggs after freezing and thawing. Over the past ten years, with the maturity of egg freezing technology, the survival rate of egg cells after freezing and thawing has been greatly improved. How to predict the survival rate of eggs after freezing and thawing is still an important challenge for clinicians.

Egg survival rate is affected by different factors, age is one of the factors. Younger eggs are of better quality and therefore may have a higher survival rate. Can AMH, a predictor of ovarian reserve, also serve as a prognostic marker for survival after egg freezing and thawing? In the research of REPRODUCTIVE BIOMEDICINE ONLINE by the Spanish IVI-RMA team in the Arab reproductive center in July 2020, according to the female AMH value, will it affect the survival rate of eggs after freezing and thawing, and the blastocyst rate of eggs after fertilization? To explore the correlation between AMH value and subsequent survival rate, fertilization rate and blastocyst rate of frozen eggs.

Correlation Analysis of Egg Survival Rate

Based on the multivariate correlation analysis between AMH value (ng/ml) and egg survival rate, the team found that there is a positive correlation between AMH value and egg survival rate. The higher the AMH, the higher the egg survival rate (Figure 1 ).

Taking age as an indicator, the age is divided into four intervals: 20-31 years old, 31-37 years old, 37-42 years old, and 42-48 years old. Taking 31-37 years old as the reference value, we explored the correlation between age and egg survival rate. It can be seen from the results that the egg survival rate of women over 37 years old drops significantly (Figure 2).

Serum AMH concentration predicts oocyte survival rate

Use the ROC curve to predict the survival rate of eggs after freezing and thawing according to the AMH concentration. When the serum AMH concentration is lower than 1.09ng/ml, the survival rate may be lower than 70%.

Is there a difference between fresh fertilization and thawed egg fertilization?

Comparing the development results of ICSI embryos fertilized by fresh fertilized and thawed eggs of the same woman in the same cycle, observing the comparison of the number of 2PN embryos, blastocysts, fertilization rate and blastocyst rate, as shown in the table in Figure 3, There was no significant difference in the fertilization rate between fresh (70.3%) and fertilized eggs (66.1%), however, the rate of blastocyst formation after fertilization of fresh eggs was significantly higher (66.8% v.s. 52.6%).

In view of the increasing trend of egg freezing, it is crucial for each reproductive center to provide each client with the most personalized medical treatment, and to give appropriate strategies and consultations. From the conclusion of this article, it can be known that AMH in serum assess egg viability after vitrification and thawing, providing an important prognostic indicator for women who need egg freezing and their healthcare teams.

We Stork adopts a personalized treatment course and designs the most suitable course of treatment according to each woman's condition. There are many links with this article:

1. The most suitable ovulation induction treatment course is given to each woman according to the level of AMH value.

2. We Stork uses the age of 37 as the cut-off point, and it is recommended that patients over the age of 38 use the strategy of collecting blastocysts (to avoid poor survival rate of eggs after freezing and thawing).

When a woman grows older, it will not only affect the ovarian reserve, but also affect the quality of eggs, the survival rate of eggs after thawing, and even the rate of blastocysts after fertilization. If you do not have any conception plan, why not freeze the young and high-quality eggs to  ensure future conception?